Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. Sample Type:Cell and tissue culture supernatants, plasma and other biological fluids (optimized by end user).Intended Use:Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples. The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.Test Principle:Lipid Peroxidation BioAssay™ Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (y = 532nm) or fluorometrically (Ex/Em = 532/553nm). Kit Components:MDA Lysis Buffer, 1x25mlPhosphotungstic Acid Solution, 1x12.5mlBHT (100X), 1x1mlTBA, 4x bottlesMDA Standard, 4.17M, 1x100ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Catalog ID||167901-100RXN||Supplier Catalog ID||167901|
|Size||100 Reactions||Price||$ 766.00|
|Supplier||United States Biological Inc.||Package Content||Lipid Peroxidation (MDA) BioAssay™ Kit (Colorimetric, Fluorometric), 100Tests|
|Shipping Temperature||RT||Storage & Handling||-20°C|
For research use only.