Specifications

Variant Details
Catalog IDN5350-29-100ULSupplier Catalog IDN5350-29
Size100 µlPrice $ 715.00
Supplier United States Biological Inc. Package Content Mouse Anti-Bovine Nitric Oxide Synthase, Pan mAb SKU:N5350-29, 100ul
General
ClonalityMonoclonalHostMouse
ReactivityBovineCross ReactivityBovine, Rat
ApplicationsELISA, Immunocytochemistry, Western blotClone No.11C140
IsotypeIgMImmunogenHighly purified bovine brain NOS protein.
GeneNitric Oxide Synthase, PanFormLyophilized
Shipping TemperatureBlue IceStorage & Handling-20°C
Storage BufferPBS, 0.05% sodium azide.PurityAscites

Description

Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme- containing flavoproteins termed NO synthase (NOS, I-III, mol wt. ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS- III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~50%. Human, rat, mouse, and bovine eNOS/NOS-3 are ~1202-1205aa proteins.

Applications:
Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1-10ug/ml
Western Blot: 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

For research use only.