Specifications

Variant Details
Catalog IDC0026-09C-ML490-100ULSupplier Catalog IDC0026-09C-ML490
Size100 µlPrice $ 892.00
Supplier United States Biological Inc. Package Content Mouse Anti- c-erbB3 (MaxLight™490) mAb SKU:C0026-09C-ML490, 100ul
General
ClonalityMonoclonalHostMouse
ApplicationsFLISA, Flow Cytometry, Immunohistochemistry, ImmunoprecipitationClone No.3H586
ConjugatedConjugatedConjugate TypeMaxLight
Specific ConjugateMaxLight™490IsotypeIgG
ImmunogenWheat germ lectin purified c-erb B-3 protein.Purification MethodPurified by Protein A affinity chromatography.
Genec-erbB3FormLiquid
Shipping TemperatureBlue IceStorage & Handling4°C. Do Not Freeze
Storage BufferPBS, pH 7.2.PurityAffinity Purified

Description

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

c-erb B-3 protein, a 180kD glycoprotein which is a growth factor receptor with tyrosine kinase activity. c-erb B-3 is expressed widely in normal human tissues, but is overexpressed in some tumors, including those of the breast, stomach, pancreas and colon.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, FLISA and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen
Optimal dilution determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab')2 (HRP) Pab RbxMo
I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note: Applications are based on unconjugated antibody.

For research use only.