Recognizes the equine CD13 cell surface antigen, also known as Aminopeptidase N, a single-pass type II membrane protein belonging to the peptidase N family with a molecular weight of between 150-170kD.
CD13 is widely expressed by a range of cell types including all blood neutrophils, basophils, monocytes, fibroblasts, kidney epithelial cells, endothelial cells and mesenchymal stem cells, but not by T or B cells. It is involved in a broad spectrum of biological processes and is believed to be linked to a number of disease states including tumor invasion (Saiki et al. 1993).
May be used for differentiating myeloid and lymphoid linage cells in tumors of the haematopoietic system.
Suitable for use in Flow Cytometry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.