Specifications

Variant Details
Catalog ID368710-100UGSupplier Catalog ID368710
Size100 µgPrice $ 538.00
Supplier United States Biological Inc. Package Content Mouse Anti-Human DNMT3L mAb SKU:368710, 100ug
General
ClonalityMonoclonalHostMouse
ReactivityHumanCross ReactivityHuman
ApplicationsELISA, Western BlotClone No.5H4A5
IsotypeIgGImmunogenRecombinant protein fragment corresponding to aa147-386 of human DNMT3L expressed in E. coli.
GeneDNMT3LFormLiquid
Shipping TemperatureBlue IceStorage & Handling-20°C
Storage BufferPBS, 0.05% sodium azidePurityPurified

Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

For research use only.