Specifications

Variant Details
Catalog ID138568-AF-ML750-100ULSupplier Catalog ID138568-AF-ML750
Size100 µlPrice $ 672.00
Supplier United States Biological Inc. Package Content Mouse Anti-Human MART-1 (MaxLight™750) mAb SKU:138568-AF-ML750, 100ul
General
ClonalityMonoclonalHostMouse
ReactivityHumanCross ReactivityHuman
ApplicationsFLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western BlotClone No.M2-7C10
ConjugatedConjugatedConjugate TypeMaxLight
Specific ConjugateMaxLight™750IsotypeIgG
ImmunogenRecombinant protein corresponding to human MART-1.Purification MethodPurified by Protein G affinity chromatography.
GeneMART-1UniProtKBQ16655
Protein Accession No.NP_005502FormLiquid
Shipping TemperatureBlue IceStorage & Handling4°C. Do Not Freeze
Storage BufferPBS, pH 7.2. No preservative added.PurityAffinity Purified

Description

MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.

This antibody recognizes a protein doublet of 20-22kD, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is pres ent in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Applications:
Suitable for use in FLISA, Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunofluorescence: 1-2ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.

Positive Control:
SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

For research use only.