Specifications

Variant Details
Catalog ID207928-100UGSupplier Catalog ID207928
Size100 µgPrice $ 775.00
Supplier United States Biological Inc. Package Content Mouse Anti-Human TP53 (ATTO 700) mAb SKU:207928, 100ug
General
ClonalityMonoclonalHostMouse
ReactivityHumanCross ReactivityHuman
ApplicationsELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western BlotClone No.Pab 1801
ConjugatedConjugatedConjugate TypeATTO
Specific ConjugateATTO 700IsotypeIgG
ImmunogenRecombinant fusion protein corresponding to aa46-53 of human TP53.Purification MethodPurified by Protein G affinity chromatography.
GeneTP53FormLiquid
Shipping TemperatureBlue IceStorage & Handling-20°C
Storage BufferPBS, pH 7.4, 0.09% sodium azide, 50% glycerol.PurityAffinity Purified

Description

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

Applications:
Suitable for use in RIA, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot (cell lysate): 1:1000-1:2000
Immunohistochemistry (frozen/formalin/PFA-fixed paraffin-embedded sections): 1:100-1:250
Immunoprecipitation: 10ug/mg
Optimal dilutions to be determined by the researcher.

Extinction Coefficient: 120,000

Excitation (Max): 700nm

Emission (Max): 725nm

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ATTO 700 conjugates are sensitive to light.

For research use only.