Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 20-50ul labels 1x10e6 cells (PBMCs)
Optimal dilutions to be determined by the researcher.
Sp2/0 AG14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.