Specifications

Variant Details
Catalog IDC2548-90CC-100RXNSupplier Catalog IDC2548-90CC
Size100 ReactionsPrice $ 665.00
Supplier United States Biological Inc. Package Content Mouse Anti-Porcine CD163 (PE) mAb SKU:C2548-90CC, 100Tests
General
ClonalityMonoclonalHostMouse
ReactivityPorcineCross ReactivityPig
Applicable ProcessesFlow cytometryClone No.10B837
ConjugatedConjugatedConjugate TypePE (Phycoerythrin)
IsotypeIgGImmunogenPorcine alveolar macrophages.
Purification MethodPurified by Protein A affinity chromatography from tissue culture supernatant.GeneCD163
UniProtKBQ2VL90FormLyophilized
Shipping TemperatureBlue IceStorage & Handling4°C. Do Not Freeze
Storage BufferPBS, pH 7.4, 1% BSA, 5% sucrose, 0.09% sodium azide.PurityAffinity Purified

Description

CD163 is a 120kD cell surface glycoprotein that is expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag.8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

For research use only.