Specifications

Variant Details
Catalog IDC2259-12B2-100RXNSupplier Catalog IDC2259-12B2
Size100 ReactionsPrice $ 736.00
Supplier United States Biological Inc. Package Content Mouse Anti-Sheep CD8 (PE) mAb SKU:C2259-12B2, 100Tests
General
ClonalityMonoclonalHostMouse
ReactivitySheepCross ReactivityBovine, Goat, Sheep
ApplicationsFlow cytometryClone No.10B1988
ConjugatedConjugatedConjugate TypePE (Phycoerythrin)
IsotypeIgGImmunogenSheep efferent lymphocytes
Purification MethodPurified by Protein G affinity chromatography from tissue culture supernatant.GeneCD8
FormLyophilizedShipping TemperatureBlue Ice
Storage & Handling4°C. Do Not FreezeStorage BufferPBS, pH 7.4, 1% BSA, 5% sucrose, 0.09% sodium azide.
PurityAffinity Purified

Description

CD8a is a 32-34kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the a3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-84B2: IgG2a Negative Control, Murine (PE)

Hybridoma:
NS-1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at 4°C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4°C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.