MystiCq® microRNA® SYBR® Green qPCR ReadyMix™ iQ™ for Real-Time PCR – Sigma-Aldrich
Sigma-Aldrich’s MystiCq® microRNA® SYBR® Green qPCR ReadyMix™ iQ™ is designed for qPCR (quantitative polymerase chain reaction) with cDNA, and other tested templates, via thermal cycling, and a dedicated Hot-start Taq DNA Polymerase.
Due to Sigma-Aldrich protocol and components, this research kit has a regular and Singleplex classification.
Real-Time PCR Reaction Volume & Amplicon Size
Amplicons of varying size can be produced from the stated amount of nucleic acid template, as part of a 50 µl reaction volume. These specifications are facilitated by Sigma-Aldrich’s included enzyme: Hot-start Taq DNA Polymerase, with an activation requirement of appropriate time and temperature.
This Sigma-Aldrich Real-time PCR Kit additionally contains the fluorescent reporter SYBR Green I, with an applicable reporter label included as a corresponding reference dye. As a result, this research product is compatible with the Biorad iCycler iQ, BioRad iQ5, BioRad MyiQ, and other specified instruments.
Applications & Downstream Experiments
The MystiCq® microRNA® SYBR® Green qPCR ReadyMix™ iQ™ is suitable for gene expression analyses, among other research experiments and is compatible with electrophoresis or other tested methods.
qPCR Troubleshooting Tips
Quantitative PCR amplification problems can persist due to incorrect primer design or contamination of the sample. However, it is also vital to ensure each stage is performed at optimum temperature during annealing and elongation phases (stated in the protocol). Furthermore, enzyme concentration (available from the manufacturer) and fidelity should be considered when determining experiment processes and conditions.