Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. Since NO is rapidly converted to nitrite (NO²ˉ) and nitrate (NOᶟˉ), the total concentration of nitrite and nitrate is used as a quantitative measure of NO production. BioAssay™ Nitric Oxide Fluorometric Assay Kit provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. In the first step nitrate is converted to nitrite by nitrate reductase. In the second step, nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The fluorescent intensity is proportional to the total nitric oxide production. The kit has been tested with culture media, plasma, and tissue homogenates.Kit Components:N2577-05A: Assay Buffer 1x30mlN2577-05B: Enzyme Co-factors 1x1 vialN2577-05C: Nitrate Reductase 1x1 vialN2577-05D: Nitrate Standard 1x1 vialN2577-05E: DAN Reagent 1x1mlN2577-05F: Sodium Hydroxide 1x1mlN2577-05G: Microtiter Plates 2x96 wellsN2577-05H: Plate covers 1x2 coversN2577-05J: Enhancer 1x1 vialN2577-05K: Nitrite Standard 1x1 vialStorage and Stability:N2577-05A, Assay Buffer; Store at 4°C. Enzyme Co-factors, N2577-05B; Store at -20°C. After reconstitution, aliquot and store at -20°C. Keep on ice during use.N2577-05C, Nitrate Reductase: Store at -20°C. After reconstitution, aliquot and store at -20°C. Keep on ice during use. N2577-05C, Nitrate Standard: Store at -20°C. After reconstitution, store at 4°C. Do not freeze! The reconstituted standard is stable for 4 months when stored at 4°C.N2577-05E, DAN Reagent, and N2577-05F, Sodium Hydroxide: Ready to use. Store at 4°C.N2577-05G, Microwell Plate: Store at RT.N2577-05H, Plate Cover: Store at RT. N2577-05J, Enhancer: Store at -20°C. After reconstitution, store at 4°C.N2577-05K, Nitrite Standard: Store at -20°C. After reconstitution, store at 4°C. Do not freeze! The reconstituted standard is stable for 4 months when stored at 4°C.
|Catalog ID||N2577-05-192RXN||Supplier Catalog ID||N2577-05|
|Size||192 Reactions||Price||$ 681.00|
|Supplier||United States Biological Inc.||Package Content||Nitric Oxide Fluorometric BioAssay™ Kit, 2x96Tests|
|Shipping Temperature||RT||Storage & Handling||-20°C|
For research use only.