Specifications

Variant Details
Catalog ID 740410.10 Vendor Catalog ID 740410.10
Size 10 Preparations Price $ 98.00
Vendor Macherey-Nagel Inc. Package Content Buffer RES Buffer LYS Buffer NEU Buffer EQU Buffer WASH Buffer ELU RNase A (lyophilized) NucleoBond® Xtra Midi Columns incl. NucleoBond® Xtra Midi Column Filters Plastic Washers
General
Single/Multiple Target Kits DNA Product Size Midi
Targets Plasmid DNA Sample Type Culture
Sample Amount < 200 ml (high copy), < 400 ml (low copy) Organism Bacteria
Subspecies E. coli Technology Anion exchange chromatography
Time to Sample 70 min Size Range < 300 kbp
Yield 400 μg Elution Volume 5000 μl
Product Application Transfecting sensitive cells;;Sequencing;;Cloning Storage & Handling 18-25 °C
Expiry Time 24 months

Description

NucleoBond Xtra Midi for Plasmid DNA Extraction - Macherey-Nagel


Macherey-Nagel’s NucleoBond Xtra Midi is designed for extracting Plasmid DNA and excluding unwanted impurities. Compatible inputs include Culture, and equivalent sample types. Such inputs must originate from Bacteria or published organisms. Specific sub-species include E. coli and their close relatives. For further information, see Macherey-Nagel specifications below.


Efficient DNA isolation and optimal yield


NucleoBond Xtra Midi’s tailored protocol, Anion exchange chromatography, and Midi kit format all facilitate its key lab applications. According to Macherey-Nagel, these are; Transfecting sensitive cells;;Sequencing;;Cloning. For optimal results, the initial sample amount should be at least < 200 ml (high copy), < 400 ml (low copy). Ideal final concentration is attainable with an elution volume of 5000 μl, (i.e., elution buffer).


Applicable Methods/Experiments


The purified DNA sample is usable in the following methods: Sequencing, and other associated analyses.

For research use only.

Resources

Protocol

NucleoBond® Xtra

Safety

SDS - North America

SDS - North America

Brochure

NucleoBond® Finalizer

Handbook

LyseControl Visual Protocol

Article

Protocol

NucleoBond® Xtra

Safety

SDS - North America

SDS - North America

Brochure

NucleoBond® Finalizer

Handbook

LyseControl Visual Protocol

Publication