NucleoBond Xtra Midi for Plasmid DNA Extraction - Macherey-Nagel
Macherey-Nagel’s NucleoBond Xtra Midi is designed for extracting Plasmid DNA and excluding unwanted impurities. Compatible inputs include Culture, and equivalent sample types. Such inputs must originate from Bacteria or published organisms. Specific sub-species include E. coli and their close relatives. For further information, see Macherey-Nagel specifications below.
Efficient DNA isolation and optimal yield
NucleoBond Xtra Midi’s tailored protocol, Anion exchange chromatography, and Midi kit format all facilitate its key lab applications. According to Macherey-Nagel, these are; Transfecting sensitive cells;;Sequencing;;Cloning. For optimal results, the initial sample amount should be at least < 200 ml (high copy), < 400 ml (low copy). Ideal final concentration is attainable with an elution volume of 5000 μl, (i.e., elution buffer).
The purified DNA sample is usable in the following methods: Sequencing, and other associated analyses.