One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction) SKU: MB1003 for Real-Time PCR – Boster Bio
Boster Bio’s One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction) SKU: MB1003 is designed for qPCR (quantitative polymerase chain reaction) with specified nucleic acids, and other tested templates, via thermal cycling, and a dedicated polymerase.
Due to Boster Bio protocol and components, this research kit has a regular and Singleplex classification.
Real-Time PCR Reaction Volume & Amplicon Size
Amplicons of varying size can be produced from the stated amount of nucleic acid template, as part of a recommended reaction volume. These specifications are facilitated by Boster Bio’s included enzyme: polymerase, with an activation requirement of appropriate time and temperature.
This Boster Bio Real-time PCR Kit additionally contains the fluorescent reporter specified in the product insert, with an applicable reporter label included as a corresponding reference dye. As a result, this research product is compatible with the majority of qPCR systems, and other specified instruments.
Applications & Downstream Experiments
The One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction) SKU: MB1003 is suitable for gene expression analyses, among other research experiments and is compatible with electrophoresis or other tested methods.
qPCR Troubleshooting Tips
Quantitative PCR amplification problems can persist due to incorrect primer design or contamination of the sample. However, it is also vital to ensure each stage is performed at optimum temperature during annealing and elongation phases (stated in the protocol). Furthermore, enzyme concentration (available from the manufacturer) and fidelity should be considered when determining experiment processes and conditions.