Description

OZ Biosciences ONPG assay kit is ideal to quantify high expression level of beta-Gal. The levels of active beta-Gal expression can be quickly measured by its catalytic hydrolysis of ONPG (o-nitrophenyl-beta-D-galactopyranoside) substrate to a bright yellow product (Absorbance at 405-420nm).
LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, beta-Gal), is very stable, resistant to proteolytic degradation and easily assayed.

All the necessary reagents are provided in this assay kit and offer a rapid, simple and sensitive method to quantify spectrophotometrically the enzyme expression level in LacZ transfected cells.

- Convenient for all transfection assays
- Suitable for cultured cells and tissues
- Simple, rapid and easy-to-use
- Economical
- Optimized with stable buffers

For research use only.

Specifications

Quantification Methodβ-Galactosidase Assay
Targets/
Sensitivity/
Detection TechniqueColorimetry
Absorbance405-420 nm
Product ApplicationMonitoring of beta-Galactosidase expression level in any cell and tissue.
Imaging SystemMicro titer spectrophotometer
Storage Temperature-20 °C, 0-4 °
Expiry Time12 months
Package Content 5X Lysis Buffer Standard Dilution Buffer ONPG Substrate Solution Stop Buffer β-gal enzyme standard, 40 units
Variant Details Size: 500 Reagents Format Non-Plated Catalog ID GO10001 Price $ 207.00

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Protocol

Discussion