The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Dot Blot. Other applications not tested.Recommended Dilution: Immunofluorescence: 1:1000-1:10,000ELISA: 1:1000-1:10,000Western Blot: 1:1000-1:10000Dot Blot: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||169052-5000UG||Supplier Catalog ID||169052|
|Size||5 mg||Price||$ 415.00|
|Supplier||United States Biological Inc.||Package Content||Rabbit Anti- Glyceraldehyde-3-phosphate Dehydrogenasepolyclonal antibody SKU:169052, 5 mg|
|Cross Reactivity||Yeast||Applications||ELISA, Immunofluorescence, Western Blot|
|Immunogen||Baker’s yeast||Specificity||Recognizes Saccharomyces cerevisiae Glyceraldehyde-3-phosphate dehydrogenase.|
|Dilution||Immunofluorescence: 1:1000-1:10,000ELISA: 1:1000-1:10,000 Western Blot: 1:1000-1:10000Dot Blot: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.||Gene||Glyceraldehyde-3-phosphate Dehydrogenase|
|Form||Lyophilized||Shipping Temperature||Blue Ice|
|Storage & Handling||-20°C||Purity||Purified|
For research use only.