POU domain, class 2, transcription factor 1, also known as OTF1 or OCT1, is a protein that in humans is encoded by the POU2F1 gene. The POU2F1 transcription factor was among the first identified members of the POU transcription factor family. This gene is mapped to 1q24.1 to q24.2. It has been found that the POU dimer from the POU2F1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1 , whereas POU dimers formed on the consensus MORE (more PORE) (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) failed to interact. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA.Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions.Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||303500-100UG||Supplier Catalog ID||303500-100ug|
|Size||100 µg||Price||$ 490.00|
|Supplier||United States Biological Inc.||Package Content||Rabbit Anti-human 37165polyclonal antibody SKU:303500, 100 µg|
|Applications||Immunocytochemistry, Western Blot||Conjugated||Unconjugated|
|Isotype||IgG||Immunogen||Recombinant protein corresponding to human Oct-1 expressed in E. coli. (Position: S11-Q240).|
|Specificity||Recognizes human Oct-1.||Dilution||Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions.Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Optimal dilutions to be determined by the researcher.|
|Purification Method||Purified by immunoaffinity chromatography.||UniProtKB/Swiss-Prot||P14859|
|Form||Lyophilized||Shipping Temperature||Blue Ice|
|Storage & Handling||-20°C||Purity||Affinity Purified|
For research use only.