Specifications

Variant Details
Catalog ID303185-100UGSupplier Catalog ID303185
Size100 µgPrice $ 490.00
Supplier United States Biological Inc. Package Content Rabbit Anti-human Cathepsin D polyclonal antibody SKU:303185, 100 µg
General
ClonalityPolyclonalHostRabbit
ReactivityHumanCross ReactivityHuman
ApplicationsImmunocytochemistry, Western blotConjugatedUnconjugated
IsotypeIgGImmunogenRecombinant protein corresponding to human Cathepsin D expressed in E. coli. (Position: G65-L412).
SpecificityRecognizes human Cathepsin D.DilutionWestern Blot: 0.1-0.5ug/ml, the detection limit is ~5ng/lane under reducing conditions.Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Optimal dilutions to be determined by the researcher.
Purification MethodPurified by immunoaffinity chromatography.GeneCathepsin D
UniProtKBP07339FormLyophilized
Shipping TemperatureBlue IceStorage & Handling-20°C
PurityAffinity Purified

Description

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412aa protein with 20aa and 44aa in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~5ng/lane under reducing conditions.Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.