IL-17A (also known as interleukin-17, cytotoxic t-lymphocyte-associated 8 and CTLA-8 in rodents) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL-17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC.Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.Recommended Dilution:ELISA: 1:20,000-1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||168257-100UG||Supplier Catalog ID||168257|
|Size||100 µg||Price||$ 706.00|
|Supplier||United States Biological Inc.||Package Content||Rabbit Anti-human IL-17A polyclonal antibody SKU:168257, 100 µg|
|Applicable Processes||ELISA, Immunocytochemistry, Western blot||Conjugated||Conjugated|
|Immunogen||Recombinant protein corresponding to full length human IL-17A.||Specificity||Recognizes human IL-17A.|
|Dilution||ELISA: 1:20,000-1:100,000 Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.||Gene||IL-17A|
|Form||Lyophilized||Shipping Temperature||Blue Ice|
|Storage & Handling||-20°C||Purity||Purified|
For research use only.