Specifications

Variant Details
Catalog ID215345-50ULSupplier Catalog ID215345
Size50 µlPrice $ 569.00
Supplier United States Biological Inc. Package Content Rabbit Anti-human Internexin, alpha polyclonal antibody SKU:215345, 50 µl
General
ClonalityPolyclonalHostRabbit
ReactivityHumanCross ReactivityFeline, Human, Mouse, Rat
Applicable ProcessesImmunocytochemistry, Western blotConjugatedUnconjugated
IsotypeIgGImmunogenRecombinant protein corresponding to Internexin Alpha expressed and purified from E. coli.
SpecificityRecognizes Internexin Alpha.DilutionWestern Blot: 1:10,000-1:20,000 Immunocytochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.
GeneInternexin, alphaUniProtKBQ16352, P23565
FormLyophilizedShipping TemperatureBlue Ice
Storage & Handling-20°CPuritySerum

Description

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66kD Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.Recommended Dilution:Western Blot: 1:10,000-1:20,000 Immunocytochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.