Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene.Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.Recommended Dilution:ELISA: To detect hNeurturin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Neurturin as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant hNeurturin.ELISA (Direct): To detect hNeurturin by direct ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4ng/well of recombinant hNeurturin.Western Blot: To detect hNeurturin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNeurturin is 1.5-3ng/lane, under either reducing or non-reducing conditions.Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||143663-25UG||Supplier Catalog ID||143663|
|Size||25 µg||Price||$ 340.00|
|Supplier||United States Biological Inc.||Package Content||Rabbit Anti-human Neurturin polyclonal antibody SKU:143663, 25 µg|
|Applications||ELISA, Western Blot||Conjugated||Unconjugated|
|Specificity||Recognizes human Neurturin.||Dilution||ELISA: To detect hNeurturin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required|
|Purification Method||Purified by immunoaffinity chromatography.||Gene||Neurturin|
|Form||Lyophilized||Shipping Temperature||Blue Ice|
|Storage & Handling||-20°C||Purity||Affinity Purified|
For research use only.