Specifications

Variant Details
Catalog IDP4258-17H-HRP-100ULSupplier Catalog IDP4258-17H-HRP
Size100 µlPrice $ 865.00
Supplier United States Biological Inc. Package Content Rabbit Anti-human Platelet Derived Growth Factor BB polyclonal antibody SKU:P4258-17H-HRP, 100 µl
General
ClonalityPolyclonalHostRabbit
ReactivityHumanCross ReactivityHuman
ApplicationsELISA, Western BlotConjugatedConjugated
Conjugate TypeHRP (Horseradish Peroxidase)IsotypeIgG
ImmunogenRecombinant human PDGF-BB.SpecificityRecognizes human PDGF-BB.
DilutionELISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 0.03-0.04ug/ml Optimal dilutions to be determined by the researcher.Purification MethodPurified by immunoaffinity chromatography.
GenePlatelet Derived Growth Factor BBAdditional InformationApplications are based on unconjugated antibody.
FormLiquidShipping TemperatureBlue Ice
Storage & Handling-20°CPurityAffinity Purified

Description

PDGF-BB (Platelet derived growth factor B chain), a disulphide-linked beta-chain homodimer and member of the PDGF family, released from platelet alpha-granules following trauma/platelet activation. Naturally occurring active forms of PDGF exist as dimers of two alpha chains (AA), two beta chains (BB), or a combination of both chains (AB), all of which signal through the high-affinity PDGF-Ralpha receptor, and the latter two of which can also signal through the PDGF-Rbeta receptor. PDGFs are potent mitogens for a variety of cells including those of the connective tissue, bone and cartilage, blood and smooth muscle cells, and are involved in several biological processes including chemotaxis, hyperplasia and embryonic neurogenesis. The expression of PDGF-BB is associated with autocrine growth stimulation and the formation of various tumor types, with several studies focusing on dermatofibrosarcoma protuberans (DP), an infiltrating skin tumor arising from a translocation occurring between chromosome 22 (the location of the PDGFB gene) and chromosome 17 (the location of the COL1A1 gene), which results in the generation of the COL1A1-PDGFB chimeric protein.Applications:Suitable for use in ELISA, Functional Assays, and Western Blot. Other applications not tested. Recommended Dilution:ELISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 0.03-0.04ug/mlOptimal dilutions to be determined by the researcher. Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Note: Applications are based on unconjugated antibody.

For research use only.