A subset of the tumor necrosis factor (TNF) receptor family contain a conserved intracellular motif, the death domain. A member of this family, TRICK2, the TRAIL (TNF-related apoptosis-inducing ligand) receptor inducer of cell killing 2) is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. TRICK2, like DR4, is a receptor for TRAIL:APO-2L [2,3] and could inhibit TRAIL-induced killing.Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.Recommended Dilution:ELISA: To detect hsTRAIL Receptor-2 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human sTRAIL Receptor-2 as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant hsTRAIL Receptor-2.ELISA (Direct): To detect hsTRAIL Receptor-2 by direct ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4ng/well of recombinant hsTRAIL Receptor-2.Western Blot: To detect hsTRAIL Receptor-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL Receptor-2 is 1.5-3ng/lane, under either reducing or non-reducing conditions.Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||143687-50UG||Supplier Catalog ID||143687|
|Size||50 µg||Price||$ 446.00|
|Supplier||United States Biological Inc.||Package Content||Rabbit Anti-human sDR5 polyclonal antibody SKU:143687, 50μg|
|Applications||ELISA, Western Blot||Conjugated||Unconjugated|
|Isotype||IgG||Immunogen||human sTRAIL Receptor-2.|
|Specificity||Recognizes human sTRAIL Receptor-2.||Dilution||ELISA: To detect hsTRAIL Receptor-2 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.25-1ug/ml of this antibody is required|
|Purification Method||Purified by immunoaffinity chromatography.||Gene||sDR5|
|Form||Lyophilized||Shipping Temperature||Blue Ice|
|Storage & Handling||-20°C||Purity||Affinity Purified|
For research use only.