Specifications

Variant Details
Catalog ID208707-50UGSupplier Catalog ID208707
Size50 µgPrice $ 446.00
Supplier United States Biological Inc. Package Content Rabbit Anti-human Trefoil Factor 2 polyclonal antibody SKU:208707, 50μg
General
ClonalityPolyclonalHostRabbit
ReactivityHumanCross ReactivityHuman
ApplicationsELISA, Western blotConjugatedConjugated
Conjugate TypeBiotinIsotypeIgG
ImmunogenRecombinant protein corresponding to human TFF-2 expressed in E.coli.SpecificityRecognizes human TFF-2.
DilutionDirect ELISA: 1ug/mlPurification MethodPurified by immunoaffinity chromatography.
GeneTrefoil Factor 2FormLyophilized
Shipping TemperatureBlue IceStorage & Handling-20°C
PurityAffinity Purified

Description

Human spasmolytic polypeptide (HSP) is a trefoil peptide found prominently in the foveolar and surface epithelium, pyloric glands and mucous neck cells of the stomach. This antibody will help detect HSP in normal and uncharacterized tissues.Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.Recommended Dilution:Direct ELISA: 1ug/ml. This biotinylated polyclonal antibody allows the detection of 0.2-0.4ng/well of recombinant Human TFF-2.Sandwich ELISA: 0.25-1.0ug/ml. In conjunction with Anti-Human TECK as a capture antibody, allows the detection of 0.2-0.4ng/well of recombinant hTECK.Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible development reagents the detection limit for recombinant Human TFF-2 is 1.5-3ng/lane, under either reducing or non-reducing conditions.Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.