Human spasmolytic polypeptide (HSP) is a trefoil peptide found prominently in the foveolar and surface epithelium, pyloric glands and mucous neck cells of the stomach. This antibody will help detect HSP in normal and uncharacterized tissues.Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.Recommended Dilution:Direct ELISA: 1ug/ml. This biotinylated polyclonal antibody allows the detection of 0.2-0.4ng/well of recombinant Human TFF-2.Sandwich ELISA: 0.25-1.0ug/ml. In conjunction with Anti-Human TECK as a capture antibody, allows the detection of 0.2-0.4ng/well of recombinant hTECK.Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible development reagents the detection limit for recombinant Human TFF-2 is 1.5-3ng/lane, under either reducing or non-reducing conditions.Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||208707-50UG||Supplier Catalog ID||208707|
|Size||50 µg||Price||$ 446.00|
|Supplier||United States Biological Inc.||Package Content||Rabbit Anti-human Trefoil Factor 2 polyclonal antibody SKU:208707, 50μg|
|Applications||ELISA, Western Blot||Conjugated||Conjugated|
|Immunogen||Recombinant protein corresponding to human TFF-2 expressed in E.coli.||Specificity||Recognizes human TFF-2.|
|Dilution||Direct ELISA: 1ug/ml||Purification Method||Purified by immunoaffinity chromatography.|
|Gene||Trefoil Factor 2||Form||Lyophilized|
|Shipping Temperature||Blue Ice||Storage & Handling||-20°C|
For research use only.