Specifications

Variant Details
Catalog ID168572-100UGSupplier Catalog ID168572
Size100 µgPrice $ 621.00
Supplier United States Biological Inc. Package Content Rabbit Anti-human Tubulin, alpha polyclonal antibody SKU:168572, 100 µg
General
ClonalityPolyclonalHostRabbit
ReactivityHumanCross ReactivityBovine, Chicken, Goat, Human, Mouse, Rat, Sheep
ApplicationsELISA, Immunocytochemistry, Immunofluorescence, Western blotConjugatedConjugated
Conjugate TypeBiotinIsotypeIgG
ImmunogenSynthetic peptide corresponding to aa427-441 from human A-tubulin.SpecificityRecognizes human A-tubulin.
DilutionImmunofluorescence: 1:500-1:2000ELISA: 1:20,000-1:100,000 Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.Purification MethodPurified by immunoaffinity chromatography.
GeneTubulin, alphaFormLyophilized
Shipping TemperatureBlue IceStorage & Handling-20°C
PurityAffinity Purified

Description

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (A and B-tubulin). A and B-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric a-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated A-tubulin is referred to as G-tubulin. Another post-translational modification of detyrosinated aAtubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This makes an excellent loading control.Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.Recommended Dilution:Immunofluorescence: 1:500-1:2000ELISA: 1:20,000-1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.