Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (A and B-tubulin). A and B-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric a-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated A-tubulin is referred to as G-tubulin. Another post-translational modification of detyrosinated aAtubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This makes an excellent loading control.Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.Recommended Dilution:Immunofluorescence: 1:500-1:2000ELISA: 1:20,000-1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||168572-100UG||Supplier Catalog ID||168572|
|Size||100 µg||Price||$ 621.00|
|Supplier||United States Biological Inc.||Package Content||Rabbit Anti-human Tubulin, alpha polyclonal antibody SKU:168572, 100 µg|
|Reactivity||Human||Cross Reactivity||Bovine, Chicken, Goat, Human, Mouse, Rat, Sheep|
|Applications||ELISA, Immunocytochemistry, Immunofluorescence, Western blot||Conjugated||Conjugated|
|Immunogen||Synthetic peptide corresponding to aa427-441 from human A-tubulin.||Specificity||Recognizes human A-tubulin.|
|Dilution||Immunofluorescence: 1:500-1:2000ELISA: 1:20,000-1:100,000 Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.||Purification Method||Purified by immunoaffinity chromatography.|
|Shipping Temperature||Blue Ice||Storage & Handling||-20°C|
For research use only.