Specifications

Variant Details
Catalog ID168575-500UGSupplier Catalog ID168575
Size500 µgPrice $ 788.00
Supplier United States Biological Inc. Package Content Rabbit Anti-human Ubiquitin Activating Enzyme E1 polyclonal antibody SKU:168575, 500 µg
General
ClonalityPolyclonalHostRabbit
ReactivityHumanCross ReactivityHuman
Applicable ProcessesELISA, Immunocytochemistry, Western blotConjugatedUnconjugated
IsotypeIgGImmunogenRecombinant protein corresponding to full length human ubiquitin activating enzyme E1.
SpecificityRecognizes human ubiquitin activating enzyme E1.DilutionELISA: 1:2000-1:10,000 Western Blot: 1:1000-1:5000Immunohistochemistry: 2mg/ml-20ug/ml Optimal dilutions to be determined by the researcher.
GeneUbiquitin Activating Enzyme E1FormLyophilized
Shipping TemperatureBlue IceStorage & Handling-20°C
PurityPurified

Description

Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:1000-1:5000Immunohistochemistry: 2mg/ml-20ug/mlOptimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.