Specifications

Variant Details
Catalog ID169113-5000UGSupplier Catalog ID169113
Size5 mgPrice $ 415.00
Supplier United States Biological Inc. Package Content Rabbit Anti-porcine Isocitrate Dehydrogenasepolyclonal antibody SKU:169113, 5 mg
General
ClonalityPolyclonalHostRabbit
ReactivityPorcineCross ReactivityPig
ApplicationsELISA, Immunofluorescence, Western blotConjugatedUnconjugated
IsotypeIgGImmunogenPorcine heart
SpecificityRecognizes porcine Isocitrate Dehydrogenase.DilutionImmunofluorescence: 1:1000-1:10,000ELISA: 1:1000-1:10,000 Western Blot: 1:1000-1:10,000 Dot Blot: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.
GeneIsocitrate DehydrogenaseFormLyophilized
Shipping TemperatureBlue IceStorage & Handling-20°C
PurityPurified

Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Dot Blot. Other applications not tested.Recommended Dilution: Immunofluorescence: 1:1000-1:10,000ELISA: 1:1000-1:10,000 Western Blot: 1:1000-1:10,000 Dot Blot: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.