Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Dot Blot. Other applications not tested.Recommended Dilution: Immunofluorescence: 1:1000-1:10,000ELISA: 1:1000-1:10,000 Western Blot: 1:1000-1:10,000 Dot Blot: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||169113-5000UG||Supplier Catalog ID||169113|
|Size||5 mg||Price||$ 415.00|
|Supplier||United States Biological Inc.||Package Content||Rabbit Anti-porcine Isocitrate Dehydrogenasepolyclonal antibody SKU:169113, 5 mg|
|Applications||ELISA, Immunofluorescence, Western Blot||Conjugated||Unconjugated|
|Specificity||Recognizes porcine Isocitrate Dehydrogenase.||Dilution||Immunofluorescence: 1:1000-1:10,000ELISA: 1:1000-1:10,000 Western Blot: 1:1000-1:10,000 Dot Blot: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.|
|Shipping Temperature||Blue Ice||Storage & Handling||-20°C|
For research use only.