Perforin-1 is a protein that in humans is encoded by the PRF1 gene. Perforin is a cytolytic protein found in the granules of CD8 T-cells and NK cells. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria.Applications:Suitable for use in Western Blot. Other applications not tested.Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||138214-200UG||Supplier Catalog ID||138214|
|Size||200 µg||Price||$ 699.00|
|Supplier||United States Biological Inc.||Package Content||Rabbit Anti-rat Perforin polyclonal antibody SKU:138214, 200 µg|
|Reactivity||Rat||Cross Reactivity||Mouse, Rat|
|Isotype||IgG||Immunogen||Recombinant protein corresponding to rat perforin, expressed in E.coli.|
|Specificity||Recognizes rat perforin.||Dilution||Western Blot: 1:1000Optimal dilutions to be determined by the researcher.|
|Purification Method||Purified by Protein A affinity chromatography.||Gene||Perforin|
|Form||Lyophilized||Shipping Temperature||Blue Ice|
|Storage & Handling||-20°C||Purity||Affinity Purified|
For research use only.