Specifications

Variant Details
Catalog ID138214-200UGSupplier Catalog ID138214
Size200 µgPrice $ 699.00
Supplier United States Biological Inc. Package Content Rabbit Anti-rat Perforin polyclonal antibody SKU:138214, 200 µg
General
ClonalityPolyclonalHostRabbit
ReactivityRatCross ReactivityMouse, Rat
ApplicationsWestern blotConjugatedUnconjugated
IsotypeIgGImmunogenRecombinant protein corresponding to rat perforin, expressed in E.coli.
SpecificityRecognizes rat perforin.DilutionWestern Blot: 1:1000Optimal dilutions to be determined by the researcher.
Purification MethodPurified by Protein A affinity chromatography.GenePerforin
FormLyophilizedShipping TemperatureBlue Ice
Storage & Handling-20°CPurityAffinity Purified

Description

Perforin-1 is a protein that in humans is encoded by the PRF1 gene. Perforin is a cytolytic protein found in the granules of CD8 T-cells and NK cells. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria.Applications:Suitable for use in Western Blot. Other applications not tested.Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.