Streptavidin (MW ~66kD) binds specifically with biotin (244D). It is derived from the bacterium Streptomyces avidinii and bears a remarkable similarity to chicken egg-white avidin both in three-dimensional structure and its ability to bind biotin with extremely high affinity (Kd=10e-15M). It is a tetrameric protein capable of binding up to 4 biotin molecules. Unlike avidin, Streptavidin is non-glycosylated and is essentially neutral in charge, whereas avidin (pH 10.5) is basic at neutral pH. Because of this, streptavidin has considerably less non-specific binding resulting in less background. It has replaced avidin as the reagent of choice for most applications where protein interactions may cause background.Applications: Suitable for use in ELISA. Other applications not tested.Recommended Dilution:Optimal dilutions to be determined by the researcher.Specific Activity:15.2U/mg protein (HABA dye binding). 16.5Units/mg protein (Biotin titration)Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Catalog ID||171761-1000UG||Supplier Catalog ID||171761|
|Size||1 mg||Price||$ 373.00|
|Supplier||United States Biological Inc.||Package Content||Streptavidin (Streptomyces avidinii), 1mg|
|Storage & Handling||-20°C|
For research use only.