Variant Details
Catalog ID Vendor Catalog ID
Size 1 Kit Price $ 657.35
Vendor VWR International, LLC Package Content Supermix 1250×20 µl Reactions 10×1.25Ml SKU: 95056-500


Supermix 1250×20 µl Reactions 10×1.25Ml SKU: 95056-500 for Real-Time PCR – Quantabio

Quantabio’s Supermix 1250×20 µl Reactions 10×1.25Ml SKU: 95056-500 is designed for qPCR (quantitative polymerase chain reaction) with specified nucleic acids, and other tested templates, via thermal cycling, and a dedicated polymerase.
Due to Quantabio protocol and components, this research kit has a regular and Singleplex classification.

Real-Time PCR Reaction Volume & Amplicon Size

Amplicons of varying size can be produced from the stated amount of nucleic acid template, as part of a recommended reaction volume. These specifications are facilitated by Quantabio’s included enzyme: polymerase, with an activation requirement of appropriate time and temperature.
This Quantabio Real-time PCR Kit additionally contains the fluorescent reporter specified in the product insert, with an applicable reporter label included as a corresponding reference dye. As a result, this research product is compatible with the majority of qPCR systems, and other specified instruments.

Applications & Downstream Experiments

The Supermix 1250×20 µl Reactions 10×1.25Ml SKU: 95056-500 is suitable for gene expression analyses, among other research experiments and is compatible with electrophoresis or other tested methods.

qPCR Troubleshooting Tips

Quantitative PCR amplification problems can persist due to incorrect primer design or contamination of the sample. However, it is also vital to ensure each stage is performed at optimum temperature during annealing and elongation phases (stated in the protocol). Furthermore, enzyme concentration (available from the manufacturer) and fidelity should be considered when determining experiment processes and conditions.

For research use only.