Specifications

Variant Details
Catalog ID 95053-100 Vendor Catalog ID 101414-142
Size 1 Kit Price $ 194.60
Vendor VWR International, LLC Package Content Supermix 250×20 µl Reactions (2×1.25 Ml) SKU: 95053-100

Description

Supermix 250×20 µl Reactions (2×1.25 Ml) SKU: 95053-100 for Real-Time PCR – Quantabio


Quantabio’s Supermix 250×20 µl Reactions (2×1.25 Ml) SKU: 95053-100 is designed for qPCR (quantitative polymerase chain reaction) with specified nucleic acids, and other tested templates, via thermal cycling, and a dedicated polymerase.
Due to Quantabio protocol and components, this research kit has a regular and Singleplex classification.


Real-Time PCR Reaction Volume & Amplicon Size


Amplicons of varying size can be produced from the stated amount of nucleic acid template, as part of a recommended reaction volume. These specifications are facilitated by Quantabio’s included enzyme: polymerase, with an activation requirement of appropriate time and temperature.
This Quantabio Real-time PCR Kit additionally contains the fluorescent reporter specified in the product insert, with an applicable reporter label included as a corresponding reference dye. As a result, this research product is compatible with the majority of qPCR systems, and other specified instruments.


Applications & Downstream Experiments


The Supermix 250×20 µl Reactions (2×1.25 Ml) SKU: 95053-100 is suitable for gene expression analyses, among other research experiments and is compatible with electrophoresis or other tested methods.


qPCR Troubleshooting Tips


Quantitative PCR amplification problems can persist due to incorrect primer design or contamination of the sample. However, it is also vital to ensure each stage is performed at optimum temperature during annealing and elongation phases (stated in the protocol). Furthermore, enzyme concentration (available from the manufacturer) and fidelity should be considered when determining experiment processes and conditions.

For research use only.