Specifications

Variant Details
Catalog IDE-3061Supplier Catalog IDE-3061
Size20000 UnitsPrice $ 50.13
Supplier Bioneer Corp. Package Content T4 DNA Ligase 20,000 U 10X reaction buffer 1 ml Instruction manual
General
Enzyme TypeLigaseEnzyme Concentration200 U/µl
Unit Definition1 Weiss unit(200 unit) of enzyme is defined as the amount of enzyme required to give 90% ligation of Hind III fragments of lambda DNA in 30 min at 16 oC in 20 uL of the assay mixture.TemplatesdsDNA, dsRNA
SourceE. coliSample TypePurified DNA, Purified RNA
Sample Amount5-10 µlProduct ApplicationLigation
Applicable ProcessesLigationShipping ConditionDry ice
Shipping Temperature-70 °CStorage & Handling-20 °C
Shelf Life24 monthsResearch TopicsBiochemistry, Molecular biology

Description

T4 DNA Ligase ligates DNA from blunt-end and cohesive-end termini (Including sticky ends for TA Cloning) as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

For research use only.

Resources

Protocol

T4 DNA Ligase

Article

Protocol

T4 DNA Ligase

Brochure

Handbook

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Validation