Human thyrostimulin ranks among the glycoprotein hormone family. These hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in human: FSH, LH, TSH, and CG. FSH, LH, and TSH, mainly expressed in the anterior pituitary, are essential for coordinated endocrine regulation in the hypothalamus-pituitary axis and show to activate specific G protein-coupled receptors in the thyroid (TSH receptor) and gonads (LH and FSH receptors), respectively. The heterodimeric glycoprotein hormones have only been identified in vertebrates and are highly conserved in organisms from primitive rayfin fish (Chondrostei) to human in both primary sequences and functional characteristics. Corticotroph-derived glycoprotein hormone (CGH), also referred to as thyrostimulin, is a noncovalent heterodimer of glycoprotein hormone alpha 2 (GPHA2) and glycoprotein hormone beta 5 (GPHB5).Recombinant A2/B5 heterodimeric glycoproteins activates human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named as thyrostimulin based on its thyroid-stimulating activity. The expression of thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism. Source:Recombinant protein corresponding to human Thyrostimulin Alpha, a single, non-glycosylated, polypeptide chain containing 120aa, fused to 14aa His-tag, expressed in E. coli.Molecular Weight: ~13.34kDSpecificity:The aa sequence of the recombinant human Thyrostimulin beta subunit is 100% homologous to the aa sequence of the human Thyrostimulin beta subunit without signal sequence. (N-terminal 24aa).AA Sequence:MRGSHHHHHH GMAS QEAVIPG CHLHPFNVTV RSDRQGTCQG SHVAQACVGH CESSAFPSRY SVLVASGYRH NITSVSQCCT ISGLKKVKVQ LQCVGSRREE LEIFTARACQCDMCRLSRYStorage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
by US Biological
Thyrostimulin Alpha, Recombinant, Human, His-Tag (GPA2, GPHA2, ZSIG51, Glycoprotein Hormone alpha-2, MGC126572) SKU: 209238
|Catalog ID||209238-10UG||Supplier Catalog ID||209238|
|Size||10 µg||Price||$ 489.00|
|Supplier||United States Biological Inc.||Package Content||Thyrostimulin Alpha, Recombinant, Human, His-Tag (GPA2, GPHA2, ZSIG51, Glycoprotein Hormone alpha-2, MGC126572), 10ug|
|Purity||~95% (RP-HPLC, SDS-PAGE)||Storage & Handling||-20°C|
|Additional Information||Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4. Reconstitute with 200ul sterile 0.1M acetate buffer, pH 4 and let the lyophilized pellet dissolve completely.||Shipping Temperature||Blue Ice|
|Source||Recombinant, E. coli|
For research use only.