Specifications

Variant Details
Catalog IDTM44700Supplier Catalog IDTM44700
Size24 ReactionsPrice N/A
Supplier N/A Package Content MDx TaqMan 2X PCR Master Mix, 350 µl Toxoplasma gondii Primer Mix, 70 µl Toxoplasma gondii Positive Control, 50 µl Nuclease-Free Water (Negative Control), 1.25 ml Product Insert
General
Pathogen NameToxoplasma gondiiPathogen TypeOther microorganisms
Control TypeNegative, PositiveSample TypeBlood
TargetsGenomic DNAOrganismOther Animals
Detection ofAnimal pathogenTime to Sample150 min
Reaction Volume20 µlElongation Temperature72 °C
Amplicon Size303 bpTechnologyPCR
Specialized ApplicationDetection of Toxoplasma gondiiStorage & Handling15-25 °C, 2-8 °C, -20 °C
Shelf Life12 months

Description

Toxoplasma gondii is a single-celled organism which causes Toxoplasmosis, the most common parasitic infection worldwide affecting animals as well as humans. Cats are the only animal in which sexual reproduction of the organism occurs, and for this reason cats are the only domestic animal which has the potential to shed T. gondii eggs. When disease does occur, it may develop following primary infection due to an inadequate immune response to stop the invasive tachyzoites or as a result of a reactivated infection due to a compromised immune system. The most common symptoms of the disease are anorexia, weight loss, lethargy, dyspnea, ocular symptoms and pyrexia. As toxoplasmosis can be transmitted to humans it represents a serious health risk for people living in close contact with infected animals. Infection is especially dangerous for people with suppressed immune systems and cancer patients as well as pregnant women.

There are 3 kits available for the detection of Toxoplasma gondii:

Toxoplasma gondii TaqMan PCR Kit: SKU TM44700
- PCR control to monitor for PCR inhibition and validate the quality
- Master Mix for the target and PCR control detection
- Primer and Probe mix
- Positive control and a negative control to confirm the integrity of the kit reagents

Toxoplasma gondii SYBR Green PCR Kit: SKU SG44700
- Master Mix for the target detection
- Control Master Mix for the PCR validation
- Primers for the specific amplification of a region of the Toxoplasma gondii genome
- Positive control and a negative control to confirm the integrity of the kit reagents

Toxoplasma gondii End-Point PCR Kit: SKU EP44700
- Master Mix and primers for the specific amplification of a region of the Toxoplasma gondii genome
- Positive control and a negative control to confirm the integrity of the kit reagents
- Loading dye and a DNA ladder to facilitate analysis of the results

For research use only.

Resources

Protocol

Toxoplasma gondii TaqMan PCR Kit, Product# TM44700

Article

Protocol

Toxoplasma gondii TaqMan PCR Kit, Product# TM44700

Safety

Brochure

Handbook

Publication

Validation