Description

The Bovine Virus Diarrhea RNA Test Kit (VetMAX™-Gold BVDV PI Detection Kit) is intended for use in the rapid, in vitro detection of bovine virus diarrhea virus (BVDV RNA) extracted from ear notches in acute and persistently infected (PI) cattle.

The VetMAX™-Gold BVDV PI Detection Kit is the first USDA-licensed real-time PCR test for the detection of BVDV in cattle. The assay is a single-tube, real-time, reverse transcription-polymerase chain reaction (RT-PCR) in which RNA is reverse transcribed into cDNA, and BVDV targets are amplified and detected in real time using fluorescent TaqMan® probes.

Have Confidence in Your Results:
• Proven repeatability and reproducibility in field studies
• Highly specific and sensitive detection of type 1 and type 2 BVDV
• Consistently detects <40 RNA copies
• Contains Xeno™ RNA internal positive control for confirmation of results
• Rapid and cost-effective method for detection of BVDV with results in ~1.5 hours
• Simple, fast workflow minimizes sample handling and reduces sample cross-contamination
• Easily automated for high-throughput processing
• Detects BVDV in ear-notch tissue
• Robust and reliable AmpliTaq Gold® DNA Polymerase and TaqMan® technology

Limitations:
• The kit is not intended for discriminating viral subtypes
• Samples should be handled as recommended to prevent degradation of any BVDV RNA that is present
• RNA extraction methods should yield RNA free of RT-PCR inhibitors, which can prevent amplification of target RNA
• Follow “good PCR practices" to prevent false positive amplifications due to contamination of test samples with PCR products

For research use only.

Specifications

Detection ofAnimal pathogen
Pathogen NameBovine Viral Diarrhea Virus
Pathogen TypeVirus
Sample TypeMammalian cells, Tissue
HostAnimals
Kit TargetsViral RNA
Product ApplicationDetection of Bovine Viral Diarrhea Virus (BVDV) from 3 mm circular ear punch
TechnologyqPCR, Reverse transcription
PCR TypeReverse transcription
ControlsIncluded
Instrument CompatibilityApplied Biosystems® 7500, 7500 Fast Real-Time PCR System (96-well)
Shipping ConditionDry ice
Storage TemperatureRoom temperature, -10 °C to -30 °C
Package Content 1 Tube RT-PCR Enzyme Mix 110 µl 25X RT-PCR Enzyme Mix 110 µl 25X BVDV Primer Probe Mix 15 µl 25X BVDV Control RNA (10,000 copies/µl) 110 µl Xeno™ RNA Control (10,000 copies/µl) 500 µl Nucleic Acid Dilution Solution Nuclease-free Water
Variant Details Size: 100 Reactions Format Plated Catalog ID 4413938 Price $ 590.00

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