Description

The Zyppy™ Plasmid Midiprep Kit is a large-scale (up to 100 µg DNA) version of the Zyppy™ Plasmid Miniprep Kit. It features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using our Fast-Spin column technology. Additionally, the innovative colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The Zyppy™ Plasmid Midiprep Kit is the fastest and simplest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.

Specifications

Single/Multiple Target KitsDNA
Extraction ofPlasmid DNA
Sample TypeCulture
OrganismBacteria
TechnologySpin column
Yield> 50 µg
Time to Sample15 min
Kit SizeMidi
SubspeciesE. coli
Sample Amount6 ml
Size Range25 kb
Elution Volume150 µl
Downstream ProcessesRestriction endonuclease digestion, DNA sequencing, Transformation, Cloning, Transfection, PCR
ProcessingManual
Storage TemperatureRoom temperature, 4-8 °C
Expiry Time12 months
Package Content 7X Lysis Buffer (Blue) Neutralization Buffer (Yellow) Endo-Wash Buffer Zyppy™ Wash Buffer (concentrate) Zyppy™ Elution Buffer Zymo-Spin™ V-E Columns Zymo-Midi Filter™ (Blue) Collection tubes
Variant Details Size: 25 Preparations Format Non-Plated Catalog ID D4025 Price $ 181.00

Alternative Products

illustra plasmidPrep Midi Flow Kit GE Life Sciences
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Resources

Protocol

Zyppy™ Plasmid Midiprep Kit protocol
www.zymoresearch.de PDF

Safety

Discussion