Zyppy™ Plasmid Midiprep Kitby Zymo Research
The Zyppy™ Plasmid Midiprep Kit is a large-scale (up to 100 µg DNA) version of the Zyppy™ Plasmid Miniprep Kit. It features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using our Fast-Spin column technology. Additionally, the innovative colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The Zyppy™ Plasmid Midiprep Kit is the fastest and simplest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.