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Zyppy™ Plasmid Midiprep Kit

from Zymo Research
Size: 25 PreparationsPrice: 181.00 $ (7.24 $ / Preparation)SKU: D4025
Size: 50 PreparationsPrice: 325.00 $ (6.50 $ / Preparation)SKU: D4026
List price: 181.00 $ (25 Preparations)
list price(with free shipping)181.00* $
from Zymo Research Corporation
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Description

The Zyppy™ Plasmid Midiprep Kit is a large-scale (up to 100 µg DNA) version of the Zyppy™ Plasmid Miniprep Kit. It features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using our Fast-Spin column technology. Additionally, the innovative colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The Zyppy™ Plasmid Midiprep Kit is the fastest and simplest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.

Specifications

Single/Multiple Target KitsDNA
Extraction ofPlasmid DNA
Sample TypeCulture
OrganismBacteria
TechnologySpin column
Yield> 50 µg
Time to Sample15 min
Kit SizeMidi
High-throughput CompatibleNot specified
Low Hazard ProductNo
SubspeciesE. coli
Sample Amount6 ml
Size Range25 kb
Elution Volume150 µl
Downstream ProcessesRestriction endonuclease digestion, DNA sequencing, Transformation, Cloning, Transfection, PCR
ProcessingManual
Storage TemperatureRoom temperature, 4-8 °C
Expiry Time12 months
Package Content7X Lysis Buffer (Blue)Neutralization Buffer (Yellow)Endo-Wash BufferZyppy™ Wash Buffer (concentrate)Zyppy™ Elution BufferZymo-Spin™ V-E ColumnsZymo-Midi Filter™ (Blue)Collection tubes
Variant DetailsPreparations25FormatNon-PlatedSKUD4025 (Zymo Research), D4025 (Zymo Research Corporation)Price181.00 $

Resources

Protocols

Zyppy™ Plasmid Midiprep Kit
zymoresearch.de PDF

Discussion

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